|
| Status |
Public on Mar 13, 2020 |
| Title |
Tat101wt_1 (gene) |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cell line stably transfected with TetOff and pTRE2hyg-Tat101 vectors (Tat101 contains the first and second exons of HIV-1 Tat protein regulator)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Jurkat
|
| Treatment protocol |
NA
|
| Growth protocol |
Jurkat-TetOff were maintained in RPMI with 300 ug/ml geneticin and both Jurkat-Tat72 and Jurkat-Tat101 cell lines were maintained in RPMI with 300 ug/ml geneticin 300 ug/ml hygromycin B and (BD Biosciences and Clontech, respectively)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted with Qiagen RNeasy mini columns (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Data were processed using EASANA from GenoSplice technology. GC background correction were applied to probe intensities (core), gene and exon level expression were summarized from the corrected intensities and then quantile normalized.
HuEx-1_0-st-v2.r2.pgf
HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
|
| |
|
| Submission date |
Mar 14, 2017 |
| Last update date |
Mar 13, 2020 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
pierre.delagrange@genosplice.com
|
| Organization name |
GenoSplice technology
|
| Department |
Paris Biotech Santé
|
| Street address |
29, rue du Faubourg Saint-Jacques
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE96579 |
Modifications induced by HIV-1 Tat protein in cellular splicing mechanisms |
|
| Relations |
| Alternative to |
GSM2536210 (exon-level analysis) |
