|
| Status |
Public on Nov 03, 2008 |
| Title |
72hr antiERalpha siRNA-2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7, anti-ERa siRNA treatment, 72hr
|
| Organism |
Homo sapiens |
| Characteristics |
ER+/PR+
|
| Growth protocol |
Cells are maintained routinely at 37C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, and 10ng/ml insulin prior to treatments
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAesy purification
|
| Label |
biotin
|
| Label protocol |
Affymetrix Gene Expression TP 21 (performed by HTArray Genomics Core Facility of Lawrence Berkeley National Laboratory)
|
| |
|
| Hybridization protocol |
Affymetrix Comm Hyb Setup 21 & Affymetrix Comm Wash Stain 21 (performed by HTArray Genomics Core Facility of Lawrence Berkeley National Laboratory)
|
| Scan protocol |
GeneChip HT Scanner protocol v2.2.09 (performed by HTArray Genomics Core Facility of Lawrence Berkeley National Laboratory)
|
| Description |
Gene expression data from MCF7 after 72hr anti-Eralpha siRNA knockdown
|
| Data processing |
RMA normalization with affy package in Bioconductor R
|
| |
|
| Submission date |
Jan 03, 2008 |
| Last update date |
Jan 19, 2010 |
| Contact name |
Christina Yau |
| E-mail(s) |
cyau@buckinstitute.org
|
| Phone |
415-209-2248
|
| Organization name |
Buck Institute of Age Research
|
| Lab |
Benz Lab
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (1) |
| GSE10061 |
Expression data of MCF7 cells following short-term E deprivation, ER-alpha siRNA knockdown and oxidant treatments |
|
