Mice were injected with 5 IU of PMSG 48 hours prior to sacrifice
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from fully grown oocytes with Trizol, then separated PAGE to obtain three size fractions (19-24/24-30/19-30 nt). Adapters for Solexa sequencing were ligated, then the ligated product amplified by RT-PCR. The PCR product is gel-purified for sequencing
Label
not applicable
Label protocol
not applicable
Library strategy
OTHER
Library source
transcriptomic
Library selection
other
Instrument model
Illumina Genome Analyzer
Description
19-24 nt fraction
Data processing
The 3' linker, CTGTAGGCACCATCAAT, was identified and stripped out of the raw reads using a smith-waterman based alignment algorithm.
