|
| Status |
Public on Aug 22, 2020 |
| Title |
KP8201 AML-MSCs |
| Sample type |
RNA |
| |
|
| Source name |
Endosteal stroma of the bone marrow, transplanted with AML cells
|
| Organism |
Mus musculus |
| Characteristics |
Sex: male
tissue: bone marrow
cell type: Mesenchymal stromal cells
disease state: AML
|
| Treatment protocol |
No treatment
|
| Growth protocol |
3 mice which are KP8201, KP8203 and KP8966, were transplanted with BM transduced with MLL-AF9 while 3 mice which are KP1820, KP8816 and TU1777, were transplanted with normal BM cells. When the mice became sick, they were killed and the MSCs from the crushed bones were sorted for RNA study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction and purification of RNA were performed using spin columen (RNeasy Mini kit, Qiagen) according to the manifacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Total RNA samples (~10 pg) were amplified and labelled using the Affymetrix WT Pico Reagent Kit
|
| |
|
| Hybridization protocol |
Hybridization of Affymetrix ClariomS mouse microarrays was performed as recommenden by the manufacturer
|
| Scan protocol |
The microarrays were washed and stained in an Affymetrix Fluidics station and scanned in a GC3000 scanner with G7 update, as recommended by Affymetrix
|
| Data processing |
Signal summarization was done in Partek Genomics Suite using the RMA algorithm
|
| |
|
| Submission date |
Aug 22, 2017 |
| Last update date |
Aug 22, 2020 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
ludger.klein-hitpass@uni-essen.de
|
| Phone |
+49 201 723 85552
|
| Organization name |
Institut fuer Zellbiologie
|
| Department |
Universitaetsklinikum
|
| Lab |
BioChip Lab
|
| Street address |
Virchowstr. 173
|
| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE102928 |
The role of mesenchymal stromal cells (MSC) in the development of acute myeloid leukemia |
|
