|
| Status |
Public on Feb 28, 2018 |
| Title |
Control macrophages bio replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
macrophages isolated from the brains of 6-week-old day 4 post-MHV-infection mice
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
strain: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (Jackson)
age: 6 weeks
treated with: none (no drug control)
infected with: MHV strain, rJ2.2
time point: day 4 post-MHV-infection
tissue: brain
cell type: FACS-sorted macrophages
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated ds -cDNA were prepared according to the standard Affymetrix protocol from 1 ng total RNA (3' IVT Pico Reagent Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, ds-cDNA were hybridized for 18 hr at 45C with 60 rpm rotation on Affymatrix mouse Clariom S array.
|
| Scan protocol |
Clariom S array was scanned using Affymatrix Model 3000 scanner.
|
| Description |
C2
|
| Data processing |
Partek Genomics Suite was used to analyze the data. RMA analysis was used. Anova was used to generate p-values. Genes with adjusted p-values <0.05 and a fold-change >2 was considered significant.
affymetrix-algorithm-name = sst-rma-gene-full
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
Sep 01, 2017 |
| Last update date |
Feb 28, 2018 |
| Contact name |
Dorthea Wheeler |
| E-mail(s) |
dorthea-wheeler@uiowa.edu
|
| Organization name |
University of Iowa
|
| Street address |
51 Newton Road
|
| City |
iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE103379 |
Gene expression of macrophages isolated from mouse brains |
| GSE103727 |
Gene expression of macrophages and microglia from naïve or MHV infected mouse brains |
|
