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Status |
Public on Apr 12, 2018 |
Title |
day 15-47hi C |
Sample type |
RNA |
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|
Source name |
RUES2 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: RUES2 group: day 15 CD47hi
|
Treatment protocol |
N/A
|
Growth protocol |
Directed differentiation via definitive endoderm to anterior foregut endoderm and lung progenitors. Lung progenitors were sorted on CD47 expression and differentiated in low-Wnt media to promote airway epithelial differentiation, at which point SCGB3A2+ and SCGB3A2- cells were sorted. Cells were harvested at defined timepoints between day 0 and day 36.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAzol and the Qiagen RNeasy kit according to the manufacturer's protocol.
|
Label |
biotin
|
Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
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|
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Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Human Gene 2.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
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Description |
Gene expression data from CD47hi RUES2 cells at day 15.
|
Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.36.1), using R version 2.15.1 and the Brainarray hugene20sthsentrezg R package (version 17.0.0).
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Submission date |
Sep 05, 2017 |
Last update date |
Jun 25, 2019 |
Contact name |
Boston University Microarray and Sequencing Resource |
E-mail(s) |
msrdata@bu.edu
|
Organization name |
Boston University
|
Department |
Microarray and Sequencing Resource
|
Street address |
72 East Concord Street, E631
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
|
|
Platform ID |
GPL17930 |
Series (1) |
GSE103517 |
Kinetic microarray of hPSC-derived SCGB3A2+ airway epithelium |
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