|
| Status |
Public on Feb 14, 2018 |
| Title |
OB_WT_18M_R2 |
| Sample type |
RNA |
| |
|
| Source name |
Olfactory bulb
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
genotype/variation: wild type
tissue: Olfactory bulb
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For olfactory bulb mRNA extraction, the Maxwell 16 simplyRNA kit (Promega) was used
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was prepared from 1 ng of total RNA using the Affymetrix GeneChip® WT Pico Kit (PN902623). The sense strand cDNA was then fragmented and biotinylated according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Labeled sense cDNA was hybridized to the GeneChip® Mouse Clariom S Arrays, washed and stained on a GeneChip® Fluidics 450 workstation (Affymetrix) according to the manufacturer protocols.
|
| Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000 7G
|
| Data processing |
Data were normalized with Bioconductor software using RMA algorithm.
|
| |
|
| Submission date |
Sep 13, 2017 |
| Last update date |
Feb 14, 2018 |
| Contact name |
Victor Segura |
| E-mail(s) |
vsegura@unav.es
|
| Organization name |
FIMA
|
| Department |
Unit of Proteomics, Genomics and Bioinformatics
|
| Street address |
Avda. Pío XII, 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE103835 |
The olfactory molecular snapshot in aged Tg2576 Alzheimer´s disease mouse model |
|
