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Status |
Public on Sep 25, 2019 |
Title |
G-TPP+LXR623 2 |
Sample type |
RNA |
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Source name |
NCH644 cells treated with G-TPP+LXR623 in 0.1% DMSO
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Organism |
Homo sapiens |
Characteristics |
cell line: NCH644 cell type: stem cell like glioma cells treatment: G-TPP+LXR623
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Treatment protocol |
After one day of cell seeding, NCH644 cells were treated with 1 µM of G-TPP, 5 µM of LXR623, both, or neither (in 0.1% DMSO) for 24 hours.
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Growth protocol |
Human stem cell like glioma cells (NCH644) were cultured in GBM-MG medium (CLS, Heidelberg, Germany) at 37°C in 5% CO2.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, Hiden, Germany) according to the manufacturer's protocol.
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Label |
biotin
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Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
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Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Human Gene 2.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
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Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
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Description |
Gene expression data from NCH644 cells treated with G-TPP+LXR623 in 0.1% DMSO.
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Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.36.1), using R version 2.15.1 and the Brainarray hugene20sthsentrezg R package (version 17.0.0).
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Submission date |
Sep 26, 2017 |
Last update date |
Sep 25, 2019 |
Contact name |
Boston University Microarray and Sequencing Resource |
E-mail(s) |
msrdata@bu.edu
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Organization name |
Boston University
|
Department |
Microarray and Sequencing Resource
|
Street address |
72 East Concord Street, E631
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
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|
Platform ID |
GPL17930 |
Series (1) |
GSE104272 |
Combination effect of G-TPP and LXR623 on stem cell like glioma cells |
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