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Sample GSM2795221 Query DataSets for GSM2795221
Status Public on May 01, 2018
Title VIMN_N690_CD206-
Sample type RNA
 
Source name Primary pancreatic FACS-sorted cells from immunized vehicle treated mice
Organism Mus musculus
Characteristics age: 4 month old
tissue: Pancreatic macrophages
treatment: vehicle
Treatment protocol Animals were maintained under standard conditions, immunized with pre-proinsulin and BL001- or Vehicle-treated for 8 weeks
Growth protocol Standard feeding
Extracted molecule total RNA
Extraction protocol RNA from FACS-sorted cells, obtained from different pool of mice per group, in triplicates was isolated using the RNAesay micro kit according to the manufacturer´s protocol (Qiagen).
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 1 ng total RNA Labeling Protocol (The Ambion WT Expression kit for Affymetrix 3’-IVT Pico, Life Technologies Corporation, 2009).
 
Hybridization protocol Following fragmentation, 5.5 ug of double-stranded cDNA were hybridized for 14 hr at 40C on Clariom™ S Assay, Mouse. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
Scan protocol Microarrays were scanned using GeneChip® Scanner 3000 7G
Description The mouse presented healthy appearance
Data processing The .CEL files were generated using Affymetrix GCOS software and further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma package of R/Bioconductor project.
 
Submission date Sep 27, 2017
Last update date May 01, 2018
Contact name Benoit Raymond Gauthier
E-mail(s) benoit.gauthier@cabimer.es
Organization name Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER)
Department Stem Cells
Lab Pancreatic Islet Development and Regeneration Unit
Street address Americo Vespucio Avenue
City Seville
State/province Seville
ZIP/Postal code 41092
Country Spain
 
Platform ID GPL23038
Series (2)
GSE104322 LRH-1/NR5A2 induces M1 to M2 macrophage phenotypic switch
GSE104328 LRH-1/NR5A2 for the treatment of autoimmune diseases

Data table header descriptions
ID_REF
VALUE Relative gene expression value (RMA, log2)

Data table
ID_REF VALUE
ERCCmix1step1 5.576942404
ERCCmix1step10 4.512105762
ERCCmix1step11 6.48216903
ERCCmix1step12 3.826414866
ERCCmix1step13 4.03180057
ERCCmix1step14 4.478921611
ERCCmix1step15 4.061456551
ERCCmix1step16 3.490994058
ERCCmix1step17 4.223464526
ERCCmix1step18 4.060423069
ERCCmix1step19 5.724990223
ERCCmix1step2 6.757549139
ERCCmix1step20 4.558514515
ERCCmix1step21 6.39387786
ERCCmix1step22 6.649322924
ERCCmix1step3 5.030428581
ERCCmix1step4 4.676987534
ERCCmix1step5 5.02406135
ERCCmix1step6 3.272105692
ERCCmix1step7 4.470790803

Total number of rows: 28815

Table truncated, full table size 860 Kbytes.




Supplementary file Size Download File type/resource
GSM2795221_NC9_VIMN_N690_CD206-.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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