|
Status |
Public on Dec 01, 2017 |
Title |
IFNg+IL13_C13-7_1 |
Sample type |
RNA |
|
|
Source name |
IFNg+IL13_C13-7_1
|
Organism |
Mus musculus |
Characteristics |
t-cell_clone: 13-7 phenotype: IFN-g/ IL-13
|
Growth protocol |
12-7, 12-7, 13-11, 13-7, 16-8 maintained by weekly antigen restimulation with immune B cell APC; 3-10 weekly restimulation with immune splenocyte APC; all T cell clones in RPMI media with 3 ng/ml IL-7 for 48h prior to extraction
|
Extracted molecule |
total RNA |
Extraction protocol |
RNEasy with G-eliminator (Qiagen)
|
Label |
biotin
|
Label protocol |
Affymetrix WT Plus using 100ng of total RNA
|
|
|
Hybridization protocol |
hybridized 17h using the standard Affymetrix protocol
|
Scan protocol |
Affymetrix GeneChip Command Console used to generate CEL files
|
Data processing |
Imported into Partek Genomics Suite 6.6.RMA was generated using RMA background correction, quantile normalization and summarization by Median Polis Clariom_S_Mouse_HT.r1.pgf Clariom_S_Mouse_HT.r1.mps
|
|
|
Submission date |
Oct 10, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Raymond Morris Johnson |
E-mail(s) |
raymond.johnson@yale.edu
|
Organization name |
Yale University
|
Department |
Med/Infectious Diseases
|
Street address |
PO Box 208022
|
City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06520-8022 |
Country |
USA |
|
|
Platform ID |
GPL23038 |
Series (1) |
GSE104743 |
B cell presentation of Chlamydia antigen selects out protective CD4ɣ13 T cells; implications for genital tract tissue resident memory lymphocyte clusters |
|