|
| Status |
Public on Sep 15, 2008 |
| Title |
subject 17 Liver Rejector |
| Sample type |
RNA |
| |
|
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Exon Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Exon array data from child <20 yrs
|
| Data processing |
The data were analyzed with Affy Power Tools (APT) using rma-sketch normalization method.
|
| |
|
| Submission date |
May 06, 2008 |
| Last update date |
Apr 27, 2009 |
| Contact name |
Rakesh Sindhi |
| Organization name |
Children’s Hospital of Pittsburgh
|
| Department |
Hillman Center for Pediatric Transplantation
|
| Street address |
3705 Fifth Avenue
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL5188 |
| Series (2) |
| GSE11360 |
Exon-level summary data from Affymetrix Human Exon 1.0 ST array |
| GSE11361 |
Genetic variants in Major Histocompatibility Complex-linked genes Associate with Pediatric Liver Transplant Rejection |
|
| Relations |
| Alternative to |
GSM287111 |
