|
| Status |
Public on Mar 06, 2019 |
| Title |
PBS_CD206pos_MHCIIneg_3 |
| Sample type |
RNA |
| |
|
| Source name |
CD206+ MHC-II- Macrophages of the Meninges Post PBS Treatment - Biological Replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
cell type: macrophages of the meninges
condition: PBS-treated
markers: CD206+ MHC-II-
|
| Treatment protocol |
To induce subacute meningitis, adult mice at 6–8 weeks of age were infected intracerebrally (i.c.) with 1,5 × 104 plaque-forming units of r3LCMV. Survival was monitored daily. Stocks were prepared by a single passage on BHK-21 cells, and viral titers were determined by plaque formation on Vero cells.
|
| Growth protocol |
C57BL/6J (B6) were purchased from The Jackson Laboratory (Bar Harbor, ME). All mice in this study were handled in accordance with the guidelines set forth by the NIH Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CD206+ MHC-II+ or CD206+ MHC-II- macrophages were isolated from PBS or LCMV-infected mice at day 30 post-infection using a Beckman Astrios Cell Sorter. Macrophages were sorted to >95% purity based on the following markers: 7-AAD− Thy1.2−CD45.2+ CD11b+ Gr1− F4/80+ CD206+ and IAb/IEb +. FACS-sorted cells were collected in TRIzol (Invitrogen), and total RNA was extracted using PureLink RNA Micro kits (Life Technologies) per the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled on a sample-by-sample basis according to the manufacturer’s guidelines for use with the Mouse Clariom S Array (Affymetrix).
|
| |
|
| Hybridization protocol |
Labeled cRNA were hybridized to these arrays according to the manufacturer’s guidelines (Affymetrix) in blinded interleaved fashion.
|
| Scan protocol |
The Scanner 3000 (Affymetrix) was used in conjunction with Affymetrix AGCC software (v4.0) to generate one .CEL file per hybridized cRNA.
|
| Description |
CD206+ MHC-II- Macrophages of the Meninges Post PBS Treatment - Biological Replicate 3
|
| Data processing |
The Expression Console (Affymetrix) was used to generate 29,129 RMA normalized transcript expression values per .CEL file.
|
| |
|
| Submission date |
Jan 02, 2018 |
| Last update date |
Mar 06, 2019 |
| Contact name |
Kory R Johnson |
| E-mail(s) |
johnsonko@ninds.nih.gov
|
| Phone |
301-402-1956
|
| Organization name |
NINDS/NIH
|
| Department |
DIR IT & Bioinformatics
|
| Lab |
Bioinformatics Section
|
| Street address |
10/3B01, 9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE108647 |
Infection drives long-term meningeal engraftment by inflammatory monocytes that impair central nervous system immunity [II] |
| GSE108648 |
Infection drives long-term meningeal engraftment by inflammatory monocytes that impair central nervous system immunity |
|
