|
| Status |
Public on Jan 13, 2018 |
| Title |
KEA50_CD44wtTcl1tg_n1 |
| Sample type |
RNA |
| |
|
| Source name |
isolated CD5+/CD19+ cells from spleen of Tcl1 tg mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype/variation: Tcl1 tg
cell type: CD5+/CD19+ cells
age in days: 351
|
| Treatment protocol |
CD5+/CD19+ cells were freshly isolated from spleen of Tcl1 tg and CD44ΔB Tcl1 tg mice.
|
| Growth protocol |
Tcl1 tg mice and CD44ΔB tcl1 tg mice were sacrificed at end stage of disease.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Mini Extraction Kit (Qiagen) including Dnase digestion
|
| Label |
biotin
|
| Label protocol |
of total RNA was used to generate double-stranded cDNA. 15 µg of subsequently synthesized cRNA was purified and reverse transcribed into sense-strand (ss) cDNA, whereat unnatural dUTP residues were incorporated. Purified ss cDNA was fragmented using a combination of uracil DNA glycosylase (UDG) and apurinic/apyrimidinic endonuclease 1 (APE 1) followed by a terminal labeling with biotin. (Affymetrix GeneChip WT PLUS Reagent Kit User Manual)
|
| |
|
| Hybridization protocol |
3,8 µg fragmented and labeled ss cDNA were hybridized to Affymetrix Clariom S mouse arrays for 16 h at 45 ° C in a GeneChip hybridization oven 640. Hybridized arrays were washed and stained in an Affymetrix Fluidics Station FS450, controlled by the Affymetrix GeneChip Command Console v4.1.3 software.
|
| Scan protocol |
signals were measured with an Affymetrix GeneChip Scanner 3000 7G, controlled by the Affymetrix GeneChip Command Console v4.1.3 software.
|
| Description |
Genexpression data from isolated CD5+/CD19+ cells from spleen of Tcl1 tg mouse
|
| Data processing |
probe set signals in log2 scale were calculated by using the GCCN-SST-RMA algorithm with the Affymetrix GeneChip Expression Console v1.4 Software.
|
| |
|
| Submission date |
Jan 12, 2018 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Julia Christine Gutjahr |
| E-mail(s) |
j.gutjahr@salk.at
|
| Phone |
00435725525857
|
| Organization name |
SCRI-LIMCR
|
| Department |
Department of Internal Medicine III, Paracelsus Medical University
|
| Lab |
Laboratory for Immunological and Molecular Cancer Research
|
| Street address |
Müllner Hauptstraße 48
|
| City |
Salzburg |
| ZIP/Postal code |
5020 |
| Country |
Austria |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE109121 |
Expression data for Tcl1 tg mice compared to CD44ΔB Tcl1 tg mice |
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