|
| Status |
Public on Jan 31, 2020 |
| Title |
Patient 29, sample B57, Right colon |
| Sample type |
genomic |
| |
|
| Source name |
Right colon
|
| Organism |
Homo sapiens |
| Characteristics |
disease status: Synchronous colorectal cancer (SCRC)
patient pair number: 29
Sex: Female
age at diagnosis: 71
tissue: Right colon
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Not applicable.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNAs were extracted according to manufacturer's protocols.
|
| Label |
Biotin
|
| Label protocol |
MIP probe annealing and hybridization were performed according to manufacturer's protocols.
|
| |
|
| Hybridization protocol |
The digested DNA target was hybridized onto the OncoScan array and incubated at 49ºC in a GeneChip Hybridization oven 640 (Affymetrix) for 17h at 60rpm. OncoScan arrays were then washed and stained in a GeneChips Fluidics Station 450 (Affymetrix) using OncoScan stain and wash reagents according to the manufacturer’s instructions.
|
| Scan protocol |
The microarrays were finally scanned with a GeneChip scanner 3000 (Affymetrix).
|
| Description |
Pac29_B57
|
| Data processing |
CEL files were processed using the Affymetrix OncoScan console (version 1.3) and the Affymetrix ChAS software (version 3.2.0.1252).
|
| |
|
| Submission date |
Feb 01, 2018 |
| Last update date |
Jan 31, 2020 |
| Contact name |
Juan Luis Garcia |
| E-mail(s) |
jlgarcia@usal.es
|
| Phone |
+34923294812
|
| Organization name |
Centro de Investigación del Cáncer IBMCC-CIC
|
| Lab |
Laboratorio 12
|
| Street address |
Campus Miguel de Unamuno
|
| City |
Salamanca |
| State/province |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL21558 |
| Series (1) |
| GSE110026 |
Redefining synchronous colorectal cancer on the basis of clonality |
|
