|
| Status |
Public on Feb 05, 2019 |
| Title |
HCT116_Control |
| Sample type |
RNA |
| |
|
| Source name |
HCT116 colon carcinoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
cell type: colon carcinoma cells
treated with: 0.1% DMSO
|
| Treatment protocol |
After one day of cell seeding, HCT116 cells were treared with 20 uM of LXR623 for 24 hours in RPMI supplemented with 1.5% FBS.
|
| Growth protocol |
Human colon cancer cells (HCT116) were cultured in RPMI (Corning, New York, USA) supplemented with 10 % FBS at 37°C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, Hiden, Germany) according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Human Gene 2.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
| Description |
SAMPLE 1
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.36.1), using R version 2.15.1 and the Brainarray hugene20sthsentrezg R package (version 17.0.0).
|
| |
|
| Submission date |
Feb 05, 2018 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
msrdata@bu.edu
|
| Organization name |
Boston University
|
| Department |
Microarray and Sequencing Resource
|
| Street address |
72 East Concord Street, E631
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL17930 |
| Series (1) |
| GSE110151 |
Effect of LXR623, liver X receptor agonist, on colon cancer cells |
|
