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Status |
Public on Feb 05, 2019 |
Title |
U87_Conrol |
Sample type |
RNA |
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|
Source name |
U87 glioblastoma cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: U87 cell type: glioma cancer cells treated with: 0.1% DMSO
|
Treatment protocol |
After one day of cell seeding, U87 cells were treared with 20 uM of LXR623 for 24 hours in DMEM supplemented with 1.5% FBS.
|
Growth protocol |
Human glioma cells (HCT116) were cultured in DMEM (Corning, New York, USA) supplemented with 10 % FBS at 37°C in 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, Hiden, Germany) according to the manufacturer's protocol.
|
Label |
biotin
|
Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
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Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Human Gene 2.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
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Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
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Description |
SAMPLE 1
|
Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.36.1), using R version 2.15.1 and the Brainarray hugene20sthsentrezg R package (version 17.0.0).
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Submission date |
Feb 05, 2018 |
Last update date |
Jun 25, 2019 |
Contact name |
Boston University Microarray and Sequencing Resource |
E-mail(s) |
msrdata@bu.edu
|
Organization name |
Boston University
|
Department |
Microarray and Sequencing Resource
|
Street address |
72 East Concord Street, E631
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
|
|
Platform ID |
GPL17930 |
Series (1) |
GSE110152 |
Effect of LXR623, liver X receptor agonist, on glioma cells |
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