|
| Status |
Public on Jan 04, 2021 |
| Title |
150 gene |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer tissue
|
| Organism |
Homo sapiens |
| Characteristics |
condition: Non-Inflammatory
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy (Qiagen)
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA were amplified and labbelled according Affymetrix recommandations. Briefly, total RNA were reverse transcribed, and amplified durintg an in vitro transcription step using the WT expression Kit (Ambion). Complementary RNA were converted into sense-oriented cDNA and molecules were fragmented and labeled using the Affymetrix® GeneChip® WT Terminal Labeling Kit. 5,5µg of sens DNA were fragmented and labelled prior hybridization.
|
| |
|
| Hybridization protocol |
Human Exon 1.0 ST arrays were hybridized with 4,7µg of labelled DNA, during 17hours, at 45°C under a 60rpm rotation in Affymetrix Oven.
|
| Scan protocol |
Affymetrix 7G scanner was scanned microarrays
|
| Data processing |
Raw data were normalized and controlled with Expression console (Affymetrix).
HuEx-1_0-st-v2.r2.pgf
HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
|
| |
|
| Submission date |
Mar 06, 2018 |
| Last update date |
Jan 04, 2021 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
pierre.delagrange@genosplice.com
|
| Organization name |
GenoSplice technology
|
| Department |
Paris Biotech Santé
|
| Street address |
29, rue du Faubourg Saint-Jacques
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE111477 |
Inflammatory versus non-inflammatory breast cancers |
|
| Relations |
| Alternative to |
GSM3031931 (exon-level analysis) |
