|
| Status |
Public on Jul 30, 2008 |
| Title |
Analysis of small RNAs in murine neutrophils cultured in vitro by Solexa/Illumina genome analyzer |
| Sample type |
SRA |
| |
|
| Source name |
Murine netrophils
|
| Organism |
Mus musculus |
| Characteristics |
Cloning and high-throughput sequencing of small RNAs from neutrophils in vitro cultured from wild-type mice
|
| Treatment protocol |
in vitro cultured neutrophils from three-month-old wild-type male mice
|
| Growth protocol |
Bone marrow progenitors were isolated from wild-type mice and cultured for 6 days in SILAC-IMDM media with G-CSF and SCF. To enrich for mature neutrophils, cells were further cultured for 42 hours in SILAC-IMDM media with G-CSF and dead cells were removed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sample RNAs were isolated directly from wild-type murine neutrophils using Tri Reagent protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
To profile microRNAs in in vitro cultured murine neutrophils, small RNAs were cloned and sequenced by a high-throughput sequencing system (Solexa/Illumina genome analyzer).
|
| Data processing |
Solexa/Illumina genome analyzer
|
| |
|
| Submission date |
Jul 10, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
Whitehead Institute |
| E-mail(s) |
sgupta@wi.mit.edu
|
| Phone |
617-324-0339
|
| Organization name |
Whitehead Institute
|
| Department |
Genome Technology Core
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02141 |
| Country |
USA |
| |
|
| Platform ID |
GPL9185 |
| Series (2) |
| GSE12074 |
Analysis of small RNAs in murine neutrophils by Solexa/Illumina genome analyzer |
| GSE12075 |
The impact of microRNAs on protein output |
|
| Relations |
| SRA |
SRX029071 |
| BioSample |
SAMN00116364 |
