|
| Status |
Public on Jan 08, 2009 |
| Title |
AJR 32 |
| Sample type |
RNA |
| |
|
| Source name |
hepatic tissue, A/J, prenatal undernutrition
|
| Organism |
Mus musculus |
| Characteristics |
male 7month old offspring
|
| Treatment protocol |
pregnant mice were either treated as controls (C) and fed ad libitum for the duration of pregnancy or were treated as restricted (R) and subjected to undernutrition restriction diet. Whole mouse livers were extracted from 27 week old offspring from C or R treated mothers and flash frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total Hepatic RNA was extracted from the frozen tissue using RNeasy Mini Kits with on-column RNase-free DNase Kit digestion (Quigen)
|
| Label |
Biotin
|
| Label protocol |
standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array
|
| |
|
| Hybridization protocol |
standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array
|
| Scan protocol |
standard protocols for Affymetrix GeneChip™3000 7G laser confocal slide scanner
|
| Description |
no additional information
|
| Data processing |
standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array using Affymetrix Expression Console software
|
| |
|
| Submission date |
Jul 15, 2008 |
| Last update date |
Dec 29, 2008 |
| Contact name |
brian knight |
| E-mail(s) |
bknight@mshri.on.ca
|
| Phone |
4165868224
|
| Organization name |
samuel lunenfeld research institute
|
| Street address |
25 orde st
|
| City |
toronto |
| ZIP/Postal code |
m5s3a6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE12117 |
Role of inherent strain differences in prenatally induced differential metabolic syndrome phenotype and gene expression |
|
