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Sample GSM305568 Query DataSets for GSM305568
Status Public on Jan 08, 2009
Title B6R 7
Sample type RNA
 
Source name hepatic tissue, C57BL/6J, prenatal undernutrition
Organism Mus musculus
Characteristics male 7month old offspring
Treatment protocol pregnant mice were either treated as controls (C) and fed ad libitum for the duration of pregnancy or were treated as restricted (R) and subjected to undernutrition restriction diet. Whole mouse livers were extracted from 27 week old offspring from C or R treated mothers and flash frozen in liquid nitrogen.
Extracted molecule total RNA
Extraction protocol Total Hepatic RNA was extracted from the frozen tissue using RNeasy Mini Kits with on-column RNase-free DNase Kit digestion (Quigen)
Label Biotin
Label protocol standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array
 
Hybridization protocol standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array
Scan protocol standard protocols for Affymetrix GeneChip™3000 7G laser confocal slide scanner
Description no additional information
Data processing standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array using Affymetrix Expression Console software
 
Submission date Jul 15, 2008
Last update date Mar 04, 2014
Contact name brian knight
E-mail(s) bknight@mshri.on.ca
Phone 4165868224
Organization name samuel lunenfeld research institute
Street address 25 orde st
City toronto
ZIP/Postal code m5s3a6
Country Canada
 
Platform ID GPL6246
Series (1)
GSE12117 Role of inherent strain differences in prenatally induced differential metabolic syndrome phenotype and gene expression
Relations
Reanalyzed by GSE55559

Data table header descriptions
ID_REF
VALUE signal intensity in Affymetrix Expression Console was normalized using RMA algorithm

Data table
ID_REF VALUE
10338001 12.30595
10338003 10.82034
10338004 9.94605
10338017 13.23598
10338025 9.78817
10338026 13.45216
10338029 10.43022
10338035 9.478762
10338036 10.13685
10338037 3.506153
10338041 11.59938
10338042 10.95002
10338044 12.40423
10338047 6.90436
10338056 1.959267
10338059 13.39801
10338060 4.578347
10338063 2.74458
10338064 6.341277
10338065 6.933261

Total number of rows: 35512

Table truncated, full table size 620 Kbytes.




Supplementary file Size Download File type/resource
GSM305568.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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