|
| Status |
Public on Apr 01, 2009 |
| Title |
HUVEC-Mimic_hypoxia-probeset-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
HUVEC, mimic hypoxia, 24hrs
|
| Organism |
Homo sapiens |
| Characteristics |
Human umbilical vein endothelial cells (HUVECs) were treated with cobalt chloride of 300Μm (Sigma) for 24hrs to mimic hypoxia stress. This is one the three biological replications.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from HUVECs using TRIzol reagent according to the manufacturer’s protocols (Invitrogen, Carlsbad, CA).
|
| Label |
biotin
|
| Label protocol |
1 μg RNA of the sample was processed using the Affymetrix GeneChip® Whole Transcript Sense Target Labeling Assay (Affymetrix, Santa Clara). GeneChip® WT cDNA Synthesis Kit, WT cDNA Amplification Kit, and WT Terminal Labeling Kit (Affymetrix, Inc., Santa Clara, CA) were used for sample preparation.
|
| |
|
| Hybridization protocol |
Hybridization to HuEx1.0 microarrays was carried out following the manufacturer’s instructions. 8 μg cRNA were loaded for the second cycle cDNA reaction. Hybridization cocktails containing 3~4 μg fragmented, end-labeled cDNA were applied to GeneChip® Human Exon 1.0 ST arrays. Hybridization was performed for 16 hrs using the MES_EukGE-WS2v5_450-DEV fluidics wash and stain script.
|
| Scan protocol |
Scanning of HuEx1.0 microarrays was carried out following the manufacturer’s instructions. Arrays were scanned using the Affymetrix GCS 3000 7G and Gene-Chip Operating Software v1.3 to produce the intensity files.
|
| Description |
HUVEC-Mimic_hypoxia-probeset-rep1
|
| Data processing |
Cell intensity (CEL) file data were quantile-normalized and summarized using the Probe Logarithmic Intensity Error Estimation (PLIER) within Affymetrix Power Tools (APT).
|
| |
|
| Submission date |
Aug 26, 2008 |
| Last update date |
Jan 04, 2012 |
| Contact name |
Xingyi Hang |
| E-mail(s) |
hangxy@bmi.ac.cn
|
| Organization name |
Beijing Institute of Radiation Medicine
|
| Street address |
No. 27 Taiping Road
|
| City |
Beijing |
| ZIP/Postal code |
100850 |
| Country |
China |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE12546 |
Gene and exon expression of Human Umbilical Vein Endothelial Cell under Mimic Hypoxia Stress |
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