|
| Status |
Public on Mar 11, 2019 |
| Title |
miR-D7-1 |
| Sample type |
RNA |
| |
|
| Source name |
BMSCs D7
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow-derived mesenchymal stem cells (BMSCs)
|
| Treatment protocol |
BMSCs were cultured in osteogenic medium consisting of α-MEM supplemented with 10%FBS, 1% antibiotics, 100 nM dexamethasone, 0.2 mM ascorbic acid, and 10 mM β-glycerophosphate
|
| Growth protocol |
BMSCs were cultured in the proliferation medium with 10% FBS and 1% antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
|
| |
|
| Hybridization protocol |
DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Jun 01, 2018 |
| Last update date |
Mar 11, 2019 |
| Contact name |
Yunfei Zheng |
| E-mail(s) |
cora2005@126.com
|
| Phone |
+861082195775
|
| Organization name |
Peking University School and Hospital of Stomatology
|
| Department |
Oral and Maxillofacial Surgery
|
| Street address |
22 Zhongguancun Avenue South, Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL25134 |
| Series (1) |
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