|
| Status |
Public on Apr 01, 2009 |
| Title |
t80kd_tnf_2 |
| Sample type |
RNA |
| |
|
| Source name |
Trap-80 knock-down 3T3 fibroblasts, stimulated with TNF-alpha
|
| Organism |
Mus musculus |
| Characteristics |
transduced with shRNA targeting Trap-80 in pSuper-retro-puro
|
| Treatment protocol |
stimulated 1hr with 5ng/ml TNF-alpha
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiaquick
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling
|
| |
|
| Hybridization protocol |
according to Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling manual (version 4)
|
| Scan protocol |
according to Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling manual (version 4)
|
| Description |
stimulated Trap-80 knock-down fibroblasts, biological replicate 2
|
| Data processing |
RMA algorithm
|
| |
|
| Submission date |
Sep 08, 2008 |
| Last update date |
Apr 01, 2009 |
| Contact name |
Dominic van Essen |
| Organization name |
Institute for Research on Cancer & Aging, Nice (IRCAN)
|
| Lab |
Transcription specificity
|
| Street address |
28 Avenue Valombrose
|
| City |
Nice |
| ZIP/Postal code |
06107 |
| Country |
France |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE12697 |
Trap-80-dependence of TNF-alpha-induced genes |
|
