|
| Status |
Public on May 15, 2019 |
| Title |
Vehicle_B16-OVA_3 |
| Sample type |
RNA |
| |
|
| Source name |
B16-OVA cell line incubated with Vehicle replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: B16-OVA cell line
|
| Treatment protocol |
B16-OVA cells were incubated either with BO-112 at 0.5 µg/mL (n=3) or identical volumes of vehicle (5%glucose, n=3). After 24h, cells were collected and total RNA was isolated in two steps using TRIzol (Life technologies) and Rneasy Mini-Kit (Quiagen) purification, following manufacturer`s RNA cleanup protocol.
|
| Growth protocol |
B12-OVA cells were cultured in RPMI 1640 containing 10% fetal bovine serum, 2mM glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin, and 50 µM 2-mercaptoethanol and 400µg/mL Geneticin (Gibco).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
| Label |
Biotin
|
| Label protocol |
The sense cDNA was prepared from 300 ng of total RNA using the Ambion® WT Expression Kit. The sense strand cDNA was then fragmented and biotinylated with the Affymetrix GeneChip® WT Terminal Labeling Kit (PN 900671).
|
| |
|
| Hybridization protocol |
Labeled sense cDNA was hybridized to the Affymetrix Mouse Clariom S array according to the the manufacturer protocols and using GeneChip® Hybridization, Wash and Stain Kit.
|
| Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000.
|
| Description |
Gene expression data from whole B16-OVA cell line
|
| Data processing |
Data was normalized using Bioconductor software and RMA algorithm.
|
| |
|
| Submission date |
Jun 20, 2018 |
| Last update date |
May 15, 2019 |
| Contact name |
Maria Angela Aznar |
| E-mail(s) |
maznargo@alumni.unav.es
|
| Organization name |
Center for applied medical research (CIMA)
|
| Street address |
Pio XII num 55
|
| City |
Pamplona |
| ZIP/Postal code |
E-31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE116075 |
Immunotherapeutic effects of intratumoral nanoplexed Poly I:C (B16-OVA cell line) |
| GSE116078 |
Immunotherapeutic effects of intratumoral nanoplexed Poly I:C |
|
