|
| Status |
Public on Jul 03, 2018 |
| Title |
Run2_PBS_3 |
| Sample type |
RNA |
| |
|
| Source name |
CD11c+ cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: CD11c+ cells
cell type: Nippostrongylus brasiliensis infected lungs
|
| Treatment protocol |
Samples received either PBS or 500ng/ml recombinant RELMa treatement for 4 h at 37 deg C.
|
| Growth protocol |
CD11c+ cells isolated from Nb infected mouse lungs were plated overnight at 37 deg C and serum starved for 1 hour.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were rinsed in 1X PBS and lysed using cell lysis buffer (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Followed NanoString nCounter XT gene expression assay protocol. Briefly, hybridization buffer was mixed with the nCounter Myeloid Innate Immunity Panel reporter codeset to prepare a master mix. Master mix, sample and Rnase-fee water was combined with nCounter Myeloid Innate Immunity Panel Capture codeset. Samples were then incubated for 18 h at 65 deg C.
|
| |
|
| Hybridization protocol |
Followed NanoString nCounter XT gene expression assay protocol. Briefly, hybridization buffer was mixed with the nCounter Myeloid Innate Immunity Panel reporter codeset to prepare a master mix. Master mix, sample and Rnase-fee water was combined with nCounter Myeloid Innate Immunity Panel Capture codeset. Samples were then incubated for 18 h at 65 deg C.
|
| Scan protocol |
Raw counts of mRNA hybridized to oligos were counted using the NanoString nCounter instrument.
|
| Description |
cell lystes collected after treatment for mRNA quantification
|
| Data processing |
Data generated through applying raw files to NanoString nSolver 4.0 software.
|
| |
|
| Submission date |
Jul 02, 2018 |
| Last update date |
Jul 03, 2018 |
| Contact name |
Meera Goh Nair |
| E-mail(s) |
Meera.Nair@medsch.ucr.edu
|
| Phone |
951-827-7734
|
| Organization name |
University of California Riverside
|
| Department |
Division of Biomedical Sciences
|
| Street address |
301 School of Medicine Research Building, 900 University Avenue,
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platform ID |
GPL25266 |
| Series (1) |
| GSE116552 |
RELMa induced differential gene expression in CD11c+ cells |
|
