|
| Status |
Public on Oct 16, 2009 |
| Title |
6 h HNE+LPS, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
6 h HNE+LPS
|
| Organism |
Mus musculus |
| Characteristics |
RAW 264.7 cell line
|
| Treatment protocol |
Samples were treated with 35 μM 4-hydroxy-2-nonenal (HNE). Immediately following treatment, lipopolysaccharide (LPS) was added at a final concentration of 1 μg/mL
|
| Growth protocol |
Cells were cultured under standard incubation conditions (37 ºC, 5% CO2) and grown in RPMI supplemented with 5% FBS and 1μg/mL P/S
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested at 6 h using Versagene RNA purification and DNase treatment kits. Six wells of a 6-well plate were pooled for each biological replicate.
|
| Label |
biotin
|
| Label protocol |
10ug of cRNA is reverse transcribed to make ss cDNA of the correct sense to hybridize to the probes on the array. A total of 5.5ug of this product is fragmented, and then labeled with Terminal Deoxy Transferase with Biotin
|
| |
|
| Hybridization protocol |
Arrays were hybridized as described in the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual v4
|
| Scan protocol |
GeneChips were scanned using the The GeneChip Scanner as described in the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual v4
|
| Description |
977DWW25
|
| Data processing |
Signal intensities across microarrays were RMA normalized
|
| |
|
| Submission date |
Oct 20, 2008 |
| Last update date |
Oct 22, 2008 |
| Contact name |
David W Wright |
| E-mail(s) |
David.Wright@vanderbilt.edu
|
| Phone |
615-322-2636
|
| Fax |
615-343-1234
|
| Organization name |
Vanderbilt University
|
| Department |
Chemistry
|
| Street address |
SC Stevenson Center 7, Station B 351822
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37235-1822 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE13281 |
Expression profiles of beta-hematin (BH)- or 4-hydroxy-2-nonenal (HNE)-treated RAW 264.7 cells |
|
