|
Status |
Public on May 18, 2009 |
Title |
striatum: putamen and caudate nucleus, right, biological rep1 |
Sample type |
RNA |
|
|
Source name |
striatum: putamen and caudate nucleus, right
|
Organism |
Homo sapiens |
Characteristics |
18 w.g., case #060426 Age: 18 weeks gestation Post-Mortem Interval <1hr
|
Treatment protocol |
not applicable
|
Growth protocol |
not applicable
|
Extracted molecule |
total RNA |
Extraction protocol |
Samples were dissected from late 2nd trimester human brains obtained from the Albert Einstein College of Medicine Human Fetal Tissue Repository and total RNA isolated using TRIzol.
|
Label |
biotin
|
Label protocol |
Samples were treated with the RNeasy Mini Kit (Qiagen) to exclude smaller RNAs, and fragmented and labeled using the Affymetrix Whole Transcript Sense Target Labeling Assay.
|
|
|
Hybridization protocol |
Samples were hybridized according to Affymetrix protocols
|
Scan protocol |
Affymetrix Gene Chip Scanner 3000
|
Description |
exon-level analysis
|
Data processing |
Data were processed using Partek Genomics Suite. Core probesets were imported using RMA defaults with probe GC content correction
|
|
|
Submission date |
Oct 24, 2008 |
Last update date |
Jan 04, 2012 |
Contact name |
Matthew Bonser Johnson |
E-mail(s) |
matthew.b.johnson@yale.edu
|
Organization name |
Yale University School of Medicine
|
Department |
Neurobiology
|
Lab |
Sestan
|
Street address |
333 Cedar St., SHM C-316
|
City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06510 |
Country |
USA |
|
|
Platform ID |
GPL5188 |
Series (1) |
GSE13344 |
Exon Array expression data from 13 areas of the late second trimester human brain |
|
Relations |
Alternative to |
GSM336664 (gene-level analysis) |