|
| Status |
Public on May 18, 2009 |
| Title |
mediodorsal thalamus, left, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mediodorsal thalamus, left
|
| Organism |
Homo sapiens |
| Characteristics |
18 w.g., case #060426
Age: 18 weeks gestation
Post-Mortem Interval <1hr
|
| Treatment protocol |
not applicable
|
| Growth protocol |
not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were dissected from late 2nd trimester human brains obtained from the Albert Einstein College of Medicine Human Fetal Tissue Repository and total RNA isolated using TRIzol.
|
| Label |
biotin
|
| Label protocol |
Samples were treated with the RNeasy Mini Kit (Qiagen) to exclude smaller RNAs, and fragmented and labeled using the Affymetrix Whole Transcript Sense Target Labeling Assay.
|
| |
|
| Hybridization protocol |
Samples were hybridized according to Affymetrix protocols
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000
|
| Description |
exon-level analysis
|
| Data processing |
Data were processed using Partek Genomics Suite. Core probesets were imported using RMA defaults with probe GC content correction
|
| |
|
| Submission date |
Oct 24, 2008 |
| Last update date |
Jan 04, 2012 |
| Contact name |
Matthew Bonser Johnson |
| E-mail(s) |
matthew.b.johnson@yale.edu
|
| Organization name |
Yale University School of Medicine
|
| Department |
Neurobiology
|
| Lab |
Sestan
|
| Street address |
333 Cedar St., SHM C-316
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE13344 |
Exon Array expression data from 13 areas of the late second trimester human brain |
|
| Relations |
| Alternative to |
GSM336667 (gene-level analysis) |
