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Status |
Public on Mar 30, 2009 |
Title |
MRC-5-1 vs mix2 |
Sample type |
RNA |
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Channel 1 |
Source name |
MRC-5-1
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Organism |
Homo sapiens |
Characteristics |
Derived from normal lung tissue of a 14-week-old male fetus by J. P. Jacobs in September 1966 (Nature 227: 168-170, 1970)
|
Treatment protocol |
untreated
|
Growth protocol |
MRC-5 were cultured in DMEM (GIBICO) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone Laboratories, Logan, UT), 0.1 mM non-essential amino acids.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA are isolated from cell pellets using TriZol reagent(Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up.
|
Label |
Cy3
|
Label protocol |
Please see manufacturer's web site at http://www.agilent.com/
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Channel 2 |
Source name |
mix2
|
Organism |
Homo sapiens |
Characteristics |
mix2 are the mixture of CCD-1079sk and IMR90.
|
Treatment protocol |
untreated
|
Growth protocol |
CCD-1079sk and IMR90 were cultured in DMEM (GIBICO) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone Laboratories, Logan, UT), 0.1 mM non-essential amino acids.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA are isolated from cell pellets using TriZol reagent(Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up.
|
Label |
Cy5
|
Label protocol |
Please see manufacturer's web site at http://www.agilent.com/
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|
|
|
Hybridization protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
Scan protocol |
Please see manufacturer's web site at http://www.agilent.com/
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Description |
Please see manufacturer's web site at http://www.agilent.com/
|
Data processing |
The microarray was scanned by Agilent G2565BA scanner,raw data were extracted by Feature Extraction Software. Signal intensity were normalized using Lowess method ,GeneSpring 10.0 . VALUE:Cy3/Cy5 signal intensity, ratio:Log2 transformed
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Submission date |
Nov 12, 2008 |
Last update date |
Mar 30, 2009 |
Contact name |
Jing Liao |
E-mail(s) |
jliao@sibs.ac.cn
|
Phone |
86-21-54921387
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Fax |
86-21-54921388
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Organization name |
Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Science
|
Department |
Institute of Biochemistry and Cell Biology
|
Lab |
LeiXiao's Lab
|
Street address |
320 Yue Yang Road
|
City |
Shanghai |
ZIP/Postal code |
200031 |
Country |
China |
|
|
Platform ID |
GPL6480 |
Series (2) |
GSE13462 |
Comparison of human ES cells, mouse ES cells, rat iPS cells and their somatic cells. |
GSE13571 |
human MRC-5 cells' gene expression difference compared with that of the adult cells including CCD-1079sk and IMR90 |
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