|
| Status |
Public on Dec 02, 2008 |
| Title |
mouse cardiac fibroblast(2) |
| Sample type |
RNA |
| |
|
| Source name |
C57 ES cell adult mouse cardiac fibroblast
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6 mouse
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by RNeasy Mini kit(Qiagen)
|
| Label |
biotin
|
| Label protocol |
Labeling was performed following the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization to the Affymetrix GeneChip Gene 1.0 ST Array is performed according to standard Affymetrix protocols.
|
| Scan protocol |
After hybridization, the chips were stained and washed in a Genechip Fluidics Station 450(Affymetrix) and scanned by using a Genechip Array scanner 3000 7G (Affymetrix).
|
| Description |
mouse cardiac fibroblast cell replicate2
|
| Data processing |
The scanned data was processed using Affymetrix Expression Console version1.1 RMA Normalization and the further analysis was performed using Multi-Experiment Viewer(MEV) version 4.0
|
| |
|
| Submission date |
Dec 01, 2008 |
| Last update date |
Dec 01, 2008 |
| Contact name |
Hyun-Jai Cho |
| E-mail(s) |
hyunjaicho@snu.ac.kr
|
| Phone |
82-2-2072-3931
|
| Fax |
82-2-3675-0805
|
| Organization name |
Seoul National University Hospital
|
| Department |
Division of Cardiology, Department of Internal Medicine
|
| Street address |
28 Yongon-Dong, Jongno-gu
|
| City |
Seoul |
| ZIP/Postal code |
110-744 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE13770 |
Induction of pluripotent stem cells from adult somatic cells by protein-based dedifferentiation |
|
