|
| Status |
Public on Jun 04, 2019 |
| Title |
DX_female_774 |
| Sample type |
genomic |
| |
|
| Source name |
DX_female_774
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: brachial veins
Sex: female
breed: Dongxiang blue-shelled
|
| Growth protocol |
Regular quarantine inspection of the experimental farm in China Agricultural University was conducted.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The genomic DNA was extracted from blood samples using the avian blood DNA extraction kit (Tiangen Co., LTD., Beijing, China).. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, and agarose gel electrophoresis.
|
| Label |
RCL
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations,.
|
| Description |
Dongxiang blue-shelled chicken
|
| Data processing |
The first-step quality control and genotype calling from the raw data in the form of CEL files were performed using Affymetrix Power Tools v1.16.0 (APT) software with the criterion of dish quality control (DQC) > 0.82 and call rate > 97%. The SNP genotype was calling using Axiom™ Analysis Suite.
|
| |
|
| Submission date |
Jan 10, 2019 |
| Last update date |
Jun 06, 2019 |
| Contact name |
Xuemei Deng |
| E-mail(s) |
deng@cau.edu.cn
|
| Organization name |
China Agricultural University
|
| Department |
Department of animal breeding and genetics
|
| Lab |
National Engineering Laboratory for Animal Breeding
|
| Street address |
No.2, Yuan Ming Yuan West Road, Haidian District
|
| City |
Beijing |
| State/province |
N/A = Not Applicable |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL23815 |
| Series (1) |
| GSE124906 |
Genomic Analysis Reveals Pleiotropic Alleles at EDN3 and BMP7 Involved in Chicken Comb Color and Egg Production |
|
