|
| Status |
Public on Oct 01, 2009 |
| Title |
B16-F10 melanoma cells, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
B16F10 mouse melanoma cells, in vitro, treated by unspecific goat IgG (control), p18
|
| Organism |
Mus musculus |
| Characteristics |
B16F10 mouse melanoma cells, treated by unspecific goat IgG (control) for 2 hours, p18. This is the third biological replicate out of the four.
|
| Treatment protocol |
control goat IgG, 15 ug/ml, 2 hours, in vitro
|
| Growth protocol |
DMEM high glucose + 10% FBS + Pen/Str + glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by Qiagen RNeasy Micro Kit. The quality and quantity of the RNA was checked by Agilent Bioanalyzer and Nanodrop, respectively.
|
| Label |
Cy3
|
| Label protocol |
Agilent Dual-Color Gene Expression Protocol version 5.5
|
| |
|
| Channel 2 |
| Source name |
B16F10 mouse melanoma cells, in vitro, treated by goat polyclonal anti-mouse TIM3 antibody (RnD Systems), p18
|
| Organism |
Mus musculus |
| Characteristics |
B16F10 mouse melanoma cells, treated by polyclonal agonist goat anti-mouse TIM3 antibody for 2 hours, p18. This is the third biological replicate out of the four.
|
| Treatment protocol |
control goat IgG, 15 ug/ml, 2 hours, in vitro
|
| Growth protocol |
DMEM high glucose + 10% FBS + Pen/Str + glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by Qiagen RNeasy Micro Kit. The quality and quantity of the RNA was checked by Agilent Bioanalyzer and Nanodrop, respectively.
|
| Label |
Cy5
|
| Label protocol |
Agilent Dual-Color Gene Expression Protocol version 5.5
|
| |
|
| |
| Hybridization protocol |
Agilent Dual-Color Gene Expression Protocol version 5.5
|
| Scan protocol |
Agilent Scanner, extended dynamic range (high value: 100%, low value: 10%)
|
| Description |
Washing according to Agilent Dual-Color Gene Expression Protocol version 5.7
|
| Data processing |
Agilent Feature Extraction Software version 9, with default 4x44K settings
|
| |
|
| Submission date |
Jan 14, 2009 |
| Last update date |
Jul 22, 2009 |
| Contact name |
Zoltan Wiener |
| E-mail(s) |
wiezol@dgci.sote.hu
|
| Phone |
+3612102929
|
| Fax |
+3613036968
|
| URL |
http://www.dgci.sote.hu
|
| Organization name |
Semmelweis University
|
| Department |
Dept of Genetics, Cell- and Immunobiology
|
| Street address |
Nagyvarad ter 4.
|
| City |
Budapest |
| ZIP/Postal code |
H-1089 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE14418 |
The effect of TIM-3 stimulation on the gene expression profile of B16-F10 melanoma cells |
|
