|
| Status |
Public on Aug 30, 2019 |
| Title |
Muscle_PM-164 |
| Sample type |
RNA |
| |
|
| Source name |
Polymyositis_muscle
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Polymyositis
tissue: Muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNAeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133A Array. GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data were analyzed using GCRMA normalization.
|
| |
|
| Submission date |
Mar 18, 2019 |
| Last update date |
Sep 01, 2019 |
| Contact name |
Steven A Greenberg |
| E-mail(s) |
sagreenberg@partners.org
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Neurology
|
| Street address |
60 Fenwood Road
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
|
