|
| Status |
Public on Feb 12, 2009 |
| Title |
Lasp1-/- MEFs, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic fibroblasts, Lasp1-/-
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6NCr
genotype: Lasp1 knock out
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using a Perfect RNA Tissue kit (5 Prime Inc., Gaithersburg, MD) following manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Replicate 2 of 3.
|
| Data processing |
The data were normalized using mean/median scaling normalization and Welch t test with array track v3.3.
|
| |
|
| Submission date |
Feb 11, 2009 |
| Last update date |
Feb 11, 2009 |
| Contact name |
Han Zhang |
| E-mail(s) |
hzhang@mcg.edu
|
| Organization name |
Medical College of Georgia
|
| Department |
IMMAG
|
| Lab |
CB2701
|
| Street address |
1120 15th st
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30909 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE14800 |
Lasp1 gene disruption is linked to enhanced cell migration and tumor formation |
|
