|
| Status |
Public on Apr 03, 2020 |
| Title |
MMD_CSF_03 |
| Sample type |
RNA |
| |
|
| Source name |
cerebrospinal fluid
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cerebrospinal fluid
disease: moyamoya
|
| Extracted molecule |
total RNA |
| Extraction protocol |
With informed consent of each patient, 10ml cerebrospinal fluid was attained from lumbar puncture while following aseptic principles, and then quickly stored in a condition of -80°C. Then total RNA was extracted using miRNeasy Mini Kit (Qiagen, P/N 217004), after which quality check was performed with NanoDrop ND-2000 and Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, US).
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled and hybridized with Agilent's miRNA Complete Labeling and Hyb Kit (P/N 5190-0456).
|
| |
|
| Hybridization protocol |
Samples were labeled and hybridized with Agilent's miRNA Complete Labeling and Hyb Kit (P/N 5190-0456).
|
| Scan protocol |
Chips were scanned with Agilent Scan Control Software. For 8x60k chips, AgilentG3_miRNA mode (of resolution 3 microns) was selected.
|
| Description |
miRNA expression in cerebrospinal fluid with moyamoya disease
|
| Data processing |
Data from scanning was analyzed using Agilent Feature Extraction(AFE) Software (version 10.7.1.1).
|
| |
|
| Submission date |
Apr 15, 2019 |
| Last update date |
Apr 05, 2020 |
| Contact name |
Gang Wang |
| E-mail(s) |
wenyunyu1104@163.com
|
| Organization name |
Nanfang Hospital of Southern Medical University
|
| Department |
Neurosurgery
|
| Street address |
1023 Guangzhou Avenue North
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510515 |
| Country |
China |
| |
|
| Platform ID |
GPL25134 |
| Series (1) |
| GSE129792 |
miRNA expression in cerebrospinal fluid with moyamoya disease |
|
