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Sample GSM372447 Query DataSets for GSM372447
Status Public on May 05, 2009
Title Nonatopic subject 04, HDM stimulated CD4 T-cells
Sample type RNA
 
Source name PBMC-derived HDM-stimulated CD4 T-cells at 24 h
Organism Homo sapiens
Characteristics time point: 24 h
Treatment protocol PBMC were cultured in the presence or absence of HDM extract (CSL).
Growth protocol PBMC from cryopreserved stocks were cultured in AIM-V medium for 24 hours.
Extracted molecule total RNA
Extraction protocol At the termination of the cultures, CD4 T-cell were isolated by immunomagnetic separation (DyNAL), and total RNA was extracted by TRIzol (Invitrogen) followed by RNeasy (QIAgen).
Label biotin
Label protocol Total RNA was labeled employing the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s instructions (Affymetrix).
 
Hybridization protocol The samples were hybridized to HuGene ST1.0 microarrays according to standardized protocols from Affymetrix.
Scan protocol The samples were scanned on a GeneChip® Scanner 3000 7G (Affymetrix).
Description PBMC were from cryopreserved stocks.
Data processing The microarray data were preprocessed in Expression Consol software (Affymetrix) employing the probe logarithmic intensity error algorithm (PLIER, parameters - PM-GCBG background subtraction, quantile normalization, iterPLIER summarization).
 
Submission date Feb 19, 2009
Last update date Sep 01, 2016
Contact name Anthony Bosco
E-mail(s) Anthony.Bosco@telethonkids.org.au
Organization name Telethon Kids Institute
Street address 100 Roberts Road
City Subiaco
State/province WA
ZIP/Postal code 6008
Country Australia
 
Platform ID GPL6244
Series (1)
GSE14908 Global expression profiling of CD4 T-cell responses to house dust mite allergens in human atopics and nonatopics.
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Signal on the log2 scale calculated by Expression Consol software.

Data table
ID_REF VALUE
7896736 4.892194767
7896738 5.076950242
7896740 5.05848708
7896746 8.424516105
7896748 9.98313242
7896750 7.637489253
7896752 9.67128311
7896754 7.184549088
7896756 6.085600988
7896759 6.255363066
7896761 8.479462594
7896779 8.069127492
7896798 7.907599746
7896817 6.986085496
7896822 6.407811764
7896859 6.124263196
7896861 4.75278605
7896863 5.332895866
7896865 6.524904762
7896878 7.708539726

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM372447.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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