|
| Status |
Public on May 05, 2009 |
| Title |
Nonatopic subject 12, HDM stimulated CD4 T-cells |
| Sample type |
RNA |
| |
|
| Source name |
PBMC-derived HDM-stimulated CD4 T-cells at 24 h
|
| Organism |
Homo sapiens |
| Characteristics |
time point: 24 h
|
| Treatment protocol |
PBMC were cultured in the presence or absence of HDM extract (CSL).
|
| Growth protocol |
PBMC from cryopreserved stocks were cultured in AIM-V medium for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
At the termination of the cultures, CD4 T-cell were isolated by immunomagnetic separation (DyNAL), and total RNA was extracted by TRIzol (Invitrogen) followed by RNeasy (QIAgen).
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled employing the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
The samples were hybridized to HuGene ST1.0 microarrays according to standardized protocols from Affymetrix.
|
| Scan protocol |
The samples were scanned on a GeneChip® Scanner 3000 7G (Affymetrix).
|
| Description |
PBMC were from cryopreserved stocks.
|
| Data processing |
The microarray data were preprocessed in Expression Consol software (Affymetrix) employing the probe logarithmic intensity error algorithm (PLIER, parameters - PM-GCBG background subtraction, quantile normalization, iterPLIER summarization).
|
| |
|
| Submission date |
Feb 19, 2009 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Anthony Bosco |
| E-mail(s) |
Anthony.Bosco@telethonkids.org.au
|
| Organization name |
Telethon Kids Institute
|
| Street address |
100 Roberts Road
|
| City |
Subiaco |
| State/province |
WA |
| ZIP/Postal code |
6008 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE14908 |
Global expression profiling of CD4 T-cell responses to house dust mite allergens in human atopics and nonatopics. |
|
| Relations |
| Reanalyzed by |
GSE86357 |
