|
| Status |
Public on Jun 26, 2009 |
| Title |
Memory B cells_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Memory B cells isolated from tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tonsil
cell type: CD27-positive CD10/CD38-negative B-cells
|
| Growth protocol |
Upon magnetic cell sorting, cells were lysated in Trizol Reagent (Invitrogen) and frozen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
100 ng of RNA were labeled using the Human miRNA Microarray kit (Agilent Technologies) following the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization mix was prepared using the Human miRNA Microarray kit (Agilent Technologies) following the manufacturer's instructions. Hybridization was performed at 55C for 20h. Washing was performed using the Gene Expression Wash Buffer kit (Agilent Technologies) following the manufacturer's instructions.
|
| Scan protocol |
Scanning was performed by the Agilent Microarray Scanner and the Agilent's Scan Control Software v. A.7.0.1 (Agilent Technologies) using the XDR feature and the recommended parameter settings.
|
| Description |
Biological replicate 1 of 6.
|
| Data processing |
Raw image data were processed by the Feature Extraction software 9.5.1 (Agilent Technologies).
|
| |
|
| Submission date |
Mar 06, 2009 |
| Last update date |
Jun 26, 2009 |
| Contact name |
Katia Basso |
| E-mail(s) |
kb451@columbia.edu
|
| Phone |
212-8515269
|
| Fax |
212-8515256
|
| Organization name |
Columbia University
|
| Department |
Institute for Cancer Genetics
|
| Street address |
1150 St. Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL8227 |
| Series (1) |
| GSE15144 |
miRNA profiling of normal mature B cells |
|
