|
| Status |
Public on Jan 22, 2010 |
| Title |
control, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
normal lung tissues, control, biological rep1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal lung tissues obtained from the disease free margins with normal histology of lung cancer resection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from lung tissue using Trizol (Invitrogen) and purified using the RNeasy Mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA were prepared according to the standard Agilent protocol from 500ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.5 ug of cRNA were hybridized for 17 hr at 65C on Agilent 4x44k Whole Human Genome Array. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Agilent Scanner protocol
|
| Description |
Lung tissue samples obtained from the University of Pittsburgh Health Sciences Tissue Bank. Gene expression data from a control lung.
|
| Data processing |
We ran cyclic LOESS algorithm for all probes containing annotations for Entrez Gene ID. Then we took the average over "Probe Names" for those containing multiple replicates.
|
| |
|
| Submission date |
Mar 11, 2009 |
| Last update date |
Jan 22, 2010 |
| Contact name |
Ferhaan Ahmad |
| E-mail(s) |
ferhaan-ahmad@uiowa.edu
|
| Organization name |
University of Iowa
|
| Department |
Internal Medicine
|
| Street address |
100 Newton Road, 1191D ML
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE15197 |
RNA Expression Profiling of Lung Tissue Identifies Mutually Distinct Molecular Signatures in PAH and PH Secondary to IPF |
|
