|
| Status |
Public on Mar 24, 2009 |
| Title |
D08-1255 Hodgkin cell line U-HO1 SNP 6.0 array |
| Sample type |
genomic |
| |
|
| Source name |
Hodgkin cell line U-HO1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Hodgkin lymphoma U-HO1
|
| Treatment protocol |
None
|
| Growth protocol |
Cell lines were grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the Gentra Puregene DNA Isolation Kit (Qiagen, Hilden, Germany).
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
hybridized to 6.0 array
|
| Data processing |
Cel files were processed using the Genotyping Console software version 3.0, algorithm-name: birdseed-v2, version 2.4
CN5 data provided as a supplementary file
|
| |
|
| Submission date |
Mar 17, 2009 |
| Last update date |
Mar 23, 2009 |
| Contact name |
Inga Vater |
| E-mail(s) |
ivater@medgen.uni-kiel.de
|
| Phone |
00494315973549
|
| Fax |
00494315971880
|
| Organization name |
Univeesity hospital Schleswig-Holstein
|
| Department |
Institute of Human Genetics
|
| Street address |
Arnold-Heller-Strasse 3, Haus 10
|
| City |
Kiel |
| ZIP/Postal code |
24105 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE15264 |
TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma |
|
