organ/tissue: white blood cells tissue preparation: antibody purified sex: unknown other information: CD4+CD25negative spleen cells were purified from naive CBA/Ca mice by MACS sorting. T cells were activated overnight by solid phase anti-CD3. cDNA was preamplified (SMART) before SAGE analysis. [SAGEMap name: CD4+CD25neg activated]
Extracted molecule
total RNA
Extraction protocol
N/A
Description
Tagging_enzyme: BsmF I CGAP_Numeric_library_ID: 1063 Laboratory: Luis Graca, Sara Thompson, Chun-Yen Lin, Elizabeth Adams, Steve Cobbold, Herman Waldmann