|
Status |
Public on Jul 13, 2010 |
Title |
MCF7_normoxia_rep2 |
Sample type |
RNA |
|
|
Source name |
MCF7_normoxia
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 cell type: breast cancer growth condition: normoxia shrna: non-specific
|
Treatment protocol |
Cells were transfected with control shRNA or Reptin shRNA and grown under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions for 18 hours.
|
Growth protocol |
Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Cy3
|
Label protocol |
Refer to the Illumina Gene Expression System Manual.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Illumina BeadScan 500 System.
|
Description |
MCF7 cells transfected with non-specific shRNA and grown under Normoxic conditions.
|
Data processing |
The data were normalised using the quantile normalization method with beadarray version 1.10 and lumi version 1.8.3 in R/Bioconductor package.
|
|
|
Submission date |
Apr 08, 2009 |
Last update date |
Jul 13, 2010 |
Contact name |
Minhyung Kim |
E-mail(s) |
Minhyung.Kim@cshs.org
|
Organization name |
Cedars-Sinai Medical Center
|
Department |
Urology
|
Street address |
8700 Beverly Blvd
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90048 |
Country |
USA |
|
|
Platform ID |
GPL6947 |
Series (1) |
GSE15530 |
Genome-wide analysis of gene expression perturbed by reptin shRNA in MCF7 subjected to normoxic and hypoxic conditions |
|