|
| Status |
Public on Sep 06, 2019 |
| Title |
Blood_untrained_E1_1_28_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood, untrained, E1, 1_28, replicate 1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
gender: male
age: 56.96
participant: 8
timepoint: E1
rin-value: 8.7
cho-first-round(y/n): y
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole blood samples were collected in PAXGene Blood RNA Tubes (BD Biosciences). Total RNA including miRNAs was isolated using the PAXGene Blood miRNA kit (Qiagen) according to manufacturers recommendations.
|
| Label |
using miRNA Complete Labeling and Hyb Kit (Agilent, Cat. No 5190-0456), Cyanine 3-pcp
|
| Label protocol |
The expression profile of 2549 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations
|
| |
|
| Hybridization protocol |
Agilent protocols.
|
| Scan protocol |
Array´s were scanned with the Agilent Scanner with default settings.
|
| Description |
blood-borne miRNA sequencing
|
| Data processing |
Raw expression values were extracted using the Feature Extraction Software of Agilent. Raw signal intensities were quantil-normalized and log2 transformed.
|
| |
|
| Submission date |
Jul 06, 2019 |
| Last update date |
Sep 08, 2019 |
| Contact name |
Fabian Michael Kern |
| Organization name |
Saarland University
|
| Department |
Center for Bioinformatics
|
| Lab |
Chair for Clinical Bioinformatics
|
| Street address |
Campus E2 1
|
| City |
Saarbrücken |
| State/province |
Saarland |
| ZIP/Postal code |
66123 |
| Country |
Germany |
| |
|
| Platform ID |
GPL25134 |
| Series (1) |
| GSE133910 |
Systematic assessment of blood-borne microRNAs highlights molecular profiles of endurance sport and carbohydrate uptake |
|
