|
Status |
Public on Sep 06, 2019 |
Title |
Blood_untrained_E2_1_34_rep1 |
Sample type |
RNA |
|
|
Source name |
Peripheral blood, untrained, E2, 1_34, replicate 1
|
Organism |
Homo sapiens |
Characteristics |
tissue: whole blood gender: female age: 55.8 participant: 9 timepoint: E2 rin-value: 9.3 cho-first-round(y/n): n
|
Extracted molecule |
total RNA |
Extraction protocol |
Whole blood samples were collected in PAXGene Blood RNA Tubes (BD Biosciences). Total RNA including miRNAs was isolated using the PAXGene Blood miRNA kit (Qiagen) according to manufacturers recommendations.
|
Label |
using miRNA Complete Labeling and Hyb Kit (Agilent, Cat. No 5190-0456), Cyanine 3-pcp
|
Label protocol |
The expression profile of 2549 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations
|
|
|
Hybridization protocol |
Agilent protocols.
|
Scan protocol |
Array´s were scanned with the Agilent Scanner with default settings.
|
Description |
blood-borne miRNA sequencing
|
Data processing |
Raw expression values were extracted using the Feature Extraction Software of Agilent. Raw signal intensities were quantil-normalized and log2 transformed.
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|
|
Submission date |
Jul 06, 2019 |
Last update date |
Sep 08, 2019 |
Contact name |
Fabian Michael Kern |
Organization name |
Saarland University
|
Department |
Center for Bioinformatics
|
Lab |
Chair for Clinical Bioinformatics
|
Street address |
Campus E2 1
|
City |
Saarbrücken |
State/province |
Saarland |
ZIP/Postal code |
66123 |
Country |
Germany |
|
|
Platform ID |
GPL25134 |
Series (1) |
GSE133910 |
Systematic assessment of blood-borne microRNAs highlights molecular profiles of endurance sport and carbohydrate uptake |
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