|
| Status |
Public on Jun 19, 2009 |
| Title |
HPC-7_Scl_Chip-Seq (Scl_lane7 and Scl_lane8) |
| Sample type |
SRA |
| |
|
| Source name |
HPC-7
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Early haematopoietic cell line dervied from ES cells
|
| Growth protocol |
HPC-7 cells were grown in 10% conditioned mSCF and were routinely counted and split every day to be kept at a confluence between 0.7-2 x 106 cells per ml).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin was fixed in 0.4% formaldehyde, sheared to 200-500bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Chromatin IP against Scl protein
|
| Data processing |
Sequence data was aligned using Solexa alignment software.
|
| |
|
| Submission date |
Apr 23, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Samuel David Foster |
| E-mail(s) |
sdf21@cam.ac.uk
|
| Organization name |
Cambridge Institute for Medical Research
|
| Department |
Department of Haematology
|
| Lab |
Haematopoietic Stem Cell Lab
|
| Street address |
Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL9185 |
| Series (1) |
| GSE15806 |
The Transcriptional Programme Controlled by Scl/Tal1 Gene during Early Embryonic Haematopoietic Development |
|
| Relations |
| SRA |
SRX006799 |
| BioSample |
SAMN02197749 |
