|
Status |
Public on Jun 19, 2009 |
Title |
HPC-7_Scl_Chip-Seq (Scl_vancouver) |
Sample type |
SRA |
|
|
Source name |
HPC-7
|
Organism |
Mus musculus |
Characteristics |
cell type: Early haematopoietic cell line dervied from ES cells
|
Growth protocol |
HPC-7 cells were grown in 10% conditioned mSCF and were routinely counted and split every day to be kept at a confluence between 0.7-2 x 106 cells per ml).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Chromatin was fixed in 0.4% formaldehyde, sheared to 200-500bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer |
|
|
Description |
Chromatin IP against Scl protein
|
Data processing |
Sequence data was aligned using Eland alignment software.
|
|
|
Submission date |
Apr 23, 2009 |
Last update date |
May 15, 2019 |
Contact name |
Samuel David Foster |
E-mail(s) |
sdf21@cam.ac.uk
|
Organization name |
Cambridge Institute for Medical Research
|
Department |
Department of Haematology
|
Lab |
Haematopoietic Stem Cell Lab
|
Street address |
Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road
|
City |
Cambridge |
State/province |
Cambridgeshire |
ZIP/Postal code |
CB2 0XY |
Country |
United Kingdom |
|
|
Platform ID |
GPL9185 |
Series (1) |
GSE15806 |
The Transcriptional Programme Controlled by Scl/Tal1 Gene during Early Embryonic Haematopoietic Development |
|
Relations |
SRA |
SRX006800 |
BioSample |
SAMN02197782 |