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Sample GSM399364 Query DataSets for GSM399364
Status Public on May 02, 2009
Title T.4+8int.Th#4
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8int TCRhi
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Slide Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4+8int.Th#3, Positive Selection, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.2727
10344622 356.3565
10344624 254.898
10344633 731.9925
10344637 163.1982
10344653 23.4592
10344658 197.5482
10344674 11.4003
10344679 43.4224
10344707 254.1031
10344713 204.7108
10344723 135.4663
10344725 45.9004
10344741 3044.5331
10344743 43.22
10344750 550.3295
10344772 27.512
10344789 167.9069
10344797 445.6722
10344799 326.0257

Total number of rows: 24922

Table truncated, full table size 423 Kbytes.




Supplementary file Size Download File type/resource
GSM399364_EA07068_56650_MoGene_T.4+8int.Th_#4.cel.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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