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Sample GSM399372 Query DataSets for GSM399372
Status Public on May 02, 2009
Title T.4SP24-.Th#3
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8- TCRhi 24-/lo
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4SP24-.Th#3, CD4 Single-Positive, Mature
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 14.0913
10344622 230.243
10344624 402.4802
10344633 763.0915
10344637 238.0948
10344653 16.8056
10344658 230.5971
10344674 9.2995
10344679 38.665
10344707 214.4444
10344713 489.275
10344723 254.7921
10344725 40.8061
10344741 3481.9007
10344743 24.8642
10344750 541.3063
10344772 27.4803
10344789 158.1985
10344797 298.9892
10344799 186.7624

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399372_EA07068_52776_MoGene_T.4SP24-.Th_#3.cel.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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