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Sample GSM399385 Query DataSets for GSM399385
Status Public on May 02, 2009
Title T.8SP69+.Th#1
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4- 8+ TCRhi 69+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8SP69+.Th#1, CD8 Single-Positive, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE73864

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 25.2603
10344622 320.3342
10344624 325.3606
10344633 645.812
10344637 222.8903
10344653 14.6385
10344658 166.8206
10344674 21.6829
10344679 51.6185
10344707 228.6598
10344713 438.112
10344723 171.2496
10344725 44.478
10344741 2328.7621
10344743 26.0549
10344750 468.5251
10344772 27.1424
10344789 112.0563
10344797 398.2868
10344799 205.4228

Total number of rows: 24922

Table truncated, full table size 423 Kbytes.




Supplementary file Size Download File type/resource
GSM399385_EA07068_52771_MoGene_T.8SP69+.Th_#1.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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