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Sample GSM404004 Query DataSets for GSM404004
Status Public on May 16, 2009
Title CD4TESTCJ#3
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
genetic background: 4+ 8- B220-
phenotype markers: 4+8-B220-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description CD4 Test CJ#3
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date May 15, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 14.6534
10344622 270.1312
10344624 399.4902
10344633 711.0407
10344637 261.0109
10344653 14.9109
10344658 427.8208
10344674 9.453
10344679 34.0592
10344707 349.3294
10344713 393.9649
10344723 339.934
10344725 44.9374
10344741 2800.7735
10344743 22.8669
10344750 442.8828
10344772 30.7201
10344789 210.2298
10344797 211.3515
10344799 224.0147

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM404004_EA07068_82675_MoGene_CD4TESTCJ#3.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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